专利名称：VIRAL VECTORS AND METHODS FOR
PRODUCING AND USING THE SAME
发明人：Andrea Amalfitano,Dwight D.
Koeberl,Baodong Sun
申请号：US12976334申请日：20101222
公开号：US20110294193A1公开日：20111201
摘要：A recombinant hybrid virus, including: (a) a deleted adenovirus vector genomecomprising the adenovirus 5′ and 3′ cis-elements for viral replication and encapsidation,and further comprising a deletion in an adenovirus genomic region selected from thegroup consisting of: (i) the polymerase region, wherein said deletion essentially preventsthe expression of a functional polymerase protein from said deleted region and saidhybrid virus does not otherwise express a functional polymerase protein, (ii) the
preterminal protein region, wherein said deletion essentially prevents the expression of afunctional preterminal protein from said deleted region, and said hybrid virus does nototherwise express a functional preterminal protein, and (iii) both the regions of (i) and (ii);and (b) a recombinant adeno-associated virus (AAV) vector genome flanked by theadenovirus vector genome sequences of (a), said recombinant AAV vector genomecomprising (i) AAV 5′ and 3′ inverted terminal repeats, (ii) an AAV packaging sequence, and(iii) a heterologous nucleic acid sequence, wherein said heterologous nucleic acidsequence is flanked by the 5′ and 3′ AAV inverted terminal repeats of (i). Methods ofmaking and using the recombinant hybrid virus are also disclosed.
申请人：Andrea Amalfitano,Dwight D. Koeberl,Baodong Sun
地址：Durham NC US,Durham NC US,Morrisville NC US
国籍：US,US,US
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