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Improved transfection of eukaryontic cells with li

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专利名称:Improved transfection of eukaryontic cells

with linear polynucleotides byelectroporation

发明人:Schuler, Gerold, Prof. Dr.,Berneman, Zwi N.,

Prof.,Van Tendeloo, Viggo F.I., Dr.,Ponsaerts,Peter

申请号:EP01115076.0申请日:20010621公开号:EP1270732A1公开日:20030102

摘要:The present invention provides an improved method for gene delivery ineukaryontic cells by electroporation, preferably in human hematopoietic cells, particulardendritic cells. The method of the invention is superior to lipofection and passive pulsingof mRNA and to electroporation of plasmid cDNA for gene delivery, including tumorantigen loading of dendritic cells.

申请人:Schuler, Gerold,N.V. Antwerpes Innovatiecentrum

地址:Am Veilchenberg 25 91080 Spardorf DE,Drie Eikenstraat 661 2650 Edegem BE

国籍:DE,BE

代理机构:Helbing, Jörg, Dr. Dipl.-Chem.

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